this is an attempt to destabilize the situation among individual entrepreneurs , information was launched. again, through the internet that the markets are on strike, people do not go out. i mean those who trade there in a real situation in real life , there was not even such a situation. yes, many, uh, at that time tried to figure out how the stages of education would go on, the authorities did an active job of explaining. yes, some had a period there some day when someone there was thinking about how to live on the situation, that is, speech, even did not go about destabilizing the situation in this environment. firstly, the work of the authorities is a dialogue, and secondly, our people understand and understand, and today in this hall i saw, well, interested persons in the fair and truthful information that the heads of our ministries and state committees conveyed today, belarusian business is

faced with real racketeering in the worst traditions of the troubled times, they do not need money, but actions throughout the country, entrepreneurs receive calls. these are foreign formations of extremists. they try to ignite at any cost. the protest in belarus is at the disposal of journalists. the agency or the news turned out to be exclusive materials that reveal the entire chain. see our investigation. today. in the evening right after the panorama. those who are trying to take belarusian entrepreneurs hostage can use individual entrepreneurs as a trigger racket in the worst traditions of the time of troubles . we all have a desire to work, who benefits from the war in the ipshniks for inciting a civil conflict were belarus puppeteers and puppets of belarusian business in faces. if, in principle,

some in the markets. with such an investigation by journalists from the tv news agency, a coup at any cost, watch today right after the panorama of belarus 1. even more relevant information in the evening edition see you at 19:00.

anthrax, ulcer, scabies, rabies, hepatitis, diabetes mellitus, all these diseases can still affect animals and our pets, and, speaking with the language of agricultural animal specialists, the most important enemy remains a viral infection, which our veterinarians successfully fight only as in everything . first, the work is carried out by scientists, the virus is isolated, grown, studied, and then vaccines are created. however, let's not get ahead of ourselves and start to understand the topic in order. my name is ekaterina beretskaya. hello, and today in newcomania. we will study the recipes of modern aibolites. don't miss the next half an hour. recombinant victor and dna vaccines a new generation of vaccinations for animals to prevent bees from getting sick. what medicines scientists create for insects and solve the issue on the spot, how scientists save animals on

farms in the program very soon, the institute of experimental veterinary medicine of the academy of sciences of belarus was established back in the twenties of the last century, and in the past, present and future, of course, my colleagues asked all those involved right at the solemn event dedicated to the centenary of the institute. for a whole century, the institute of experimental veterinary medicine in belarus has been developing new methods for diagnosing and treating animal diseases. the vasilevsky institute of experimental veterinary medicine is a fairly significant scientific institution for a long period of

time, the enterprises have been quite effectively developing the production of veterinary vaccines, serum vaccines in some other areas, and they are constantly improving and interacting with our universities. first of all, local veterinary preparations are being developed and practically ensure the biological safety of our country. many of the developments are successfully sold abroad, especially in demand for vaccines against various diseases. we produce in industrial on a large scale, for example, the countries of central asia also buy belarus in shares, and as soon as a new virus that infects animals appears. our scientists are trying to find new ways to protect a vaccine or antidote. questions appeared, as if new topical infections. it's, uh, african swine fever, it's supra-bovine dermatitis . uh, other infections. here, in particular, there are problems. now on completely pathogenic influenza in the

world of birds. this is a disease that, in particular, is transmitted to humans and there is a danger infecting people, so we have all these problems at the institute. naturally, we are studying and working on these problems. plus. uh, we are doing some fundamental research for the future. here in particular. right there in our department. uh, new promising drugs that will be directed to the treatment of exactly the place that is sick, that is, pointwise they do not harm the entire body of the animal. and this is a real breakthrough in veterinary medicine.

dmitry sergeevich borisovets, head of the department of viral infections, institute of experimental veterinarians of the national academy of sciences of belarus candidate of veterinary sciences associate professor. i understand correctly that this is the same collection of deadly viruses in order to make it convenient for you to find these viruses in our animals. yes, of course, that is all for work. yes, of course at work. now i know you are creating a new generation. please tell us about combined vector and even dna vaccines, what is it for, what is the department of viral infection of the institute, experimental ventilation, for more than 50 years works on the development of vaccines for veterinary medicine, during this time more than 80 different drugs for animals have been created, and the technology that is used to create these prices. this is classic technology. that is, when we

isolate a viral pathogen, we accumulate it in cell culture, and then inactivate it, if you are a killed vaccine, and further introduce auxiliary components to form the final product, but naturally science does not stand still and new directions are developing. including where the technology is. awesome engineering, we're also moving in the same direction and in this regard, we are also developing the direction of developing new generation vaccines, this is a recombinant vaccine, a vector vaccine, a dna rna vaccine. in this regard, we work closely with the institute of biophysics and cell engineering of the national academy of sciences of belarus and the main two areas are the development of vector vaccines - these are vaccines that are being developed in relation to difficult to accumulate viruses that are difficult to cultivate. in particular. this is a respiratory sensation virus and the second direction is a marker vaccine when a specific marker. we insert viruses into the genome and such vaccines make it possible to distinguish

a vaccinated person who has been ill, this is dna. yes, but no, this is not a dna vaccine. it's a marker for a vaccine they're developing. mainly for diseases such as infectious rhinotrachitis, classical plague, swine fever, estrangement disease virus. and as for the dna of vaccines, i want it. it should be noted that technologies that work in medicine are not always acceptable for veterinary medicine. that is, for example, as shown by the covid-19 pandemic, uh, developed by dna the vaccine should be stored under conditions e, at -80 °. that is, the availability of specialized equipment is required, that is, to supply our livestock enterprises with such equipment. quite problematic, therefore, given these features of the storage of this vaccine. there are many difficulties here. okay. we have not yet explained what recommendations are, a recombinant vaccine is when we cut out a section of the nucleic acid of a virus that is responsible for the production of specific proteins, immunogenically and this

plot. we embed in some kind of viral vector . that is, as a rule, it is a safe virus that does not cause disease in animals, but it is a carrier of a nucleic acid that embeds into a cell and thus provides a whole cascade of immunological reactions that contributes to the development. respectively, i know that wild boar pigs have been found to have a new disease called hepatitis. e. in our country it is fixed and should people be afraid. we detected in the case of hepatitis during the period of the afghan war, cases were noted diseases of our servicemen and was diagnosed with hepatitis. and later, in 1981, academicians balayan was isolated from the virus from a serviceman and self-infected , as a result of which he became the discoverer of this virus. and in principle it was believed that this disease was viral hepatitis. e. it is more typical for countries with a hot climate when drinking, for example, poor-quality water. later in 2003 it was

found that this disease can be transmitted from animal to person, namely when the use of thermally poorly processed meat and liver, and wild boars from domestic pigs. and this deer and was subsequently established. what exactly is the use of termi. and poorly processed meat is one of the causes of this disease . this story can be prevented in animals. i know that you have been developing preventive measures since 2016, and we are participating in an international project within the framework of a union state. during the implementation of this project, we have developed diagnostic test systems for diagnostics for the purpose of monitoring - this is in the enzyme immunoassay in the polymet of a different chain reaction and one of the tasks. there was also the development of a system of preventive measures - this also applies to the medical economy, that is, for a person. this is more about personal hygiene. this is the consumption of well-

cooked meat and liver, and for example, if a person travels abroad, it is recommended to use bottled water, as far as agriculture is concerned, this is compliance with safety rules on the farm, and in due time for cleaning and disposal of manure, as well as disinfection and quarantine of animals when they enter the farm. and how do you detect a virus in an animal? well, as i understand it, these are the test systems. yes, some, well, just again, animals, you won’t take each one to the veterinarian , and he won’t say there, something hurts me. look yes. certainly, the peculiarity of this disease is that pigs, as a rule, get sick without malnutrition and detect clinical e, the signs of this disease are very difficult, therefore laboratory research methods, enzyme-linked immunosorbent assay of the polymer, various chain reactions, it is precisely for this purpose that we developed this system, which will allow laboratory conditions to determine e, diseased

animals, and also after this infection. that's just in the laboratory of your institute. my colleagues have gone. let's see what they saw there. in one of the laboratories of the institute of experimental veterinary medicine in belarus, scientists are studying bee diseases most important problem in this direction today. this is literally an attack on honey bees all over the world, since the seventies of the last century, the parasitic mite thieves, according to scientists, this pest poses a real threat to the existence of beekeeping, as such it complicates the immediate and episodic situation of visa bees with more than 7,000 viruses that today day more than 24 viruses are known, isolated by common and medusa. i mean, uh, the parasitic key itself. uh, besides the fact that he himself uh does a lot of damage directly. whole

branches in beekeeping, however, it delivers directly to chinese prayers and fat body direct contact, obtained with viruses. that is, in this way, they are additionally depicted by viruses. for example, according to the latest data, such a disease as the wing deformation virus is actually widespread in russia, and a case of this disease is also recorded in belarus. to be more visual , the mite represents such a flaxseed species. here you see the jar collected a large number. e tick, to slightly compare the size of the tick and the bee. i would tell you that uh, a tick that parasitizes the body in chile. it's actually a human one, but uh, a backpack about 20-24 kg on the shoulders. causes a splinter torch

, pupae and adult bees a severe disease of the gate of wasps, most of the drugs for this disease are toxic. in some cases, even bees are brought to death, our scientist managed to create an environmentally friendly, safe and mild preparations. to date, the scientific project, within the framework of which, together with the institute of fruit growing. uh, the branch laboratory of beekeeping developed the veterinary drug tolpan, here is a sample of the veterinary drug. depending on the season, it was more than 95%. now scientists are working on the creation of new equally effective means. what other viruses do you study, maybe there are some directly relevant work being done in four main areas. these are bovine

viruses, swine viruses, small ruminants and viruses carnivores. if you list all this, for example, the rabies virus of animal viruses and sheep virus is an infectious rhinotherapy. this is viral diarrhea. uh, the porcine reproductive respiratory syndrome virus is a classic brought by pigs and many others in total about 20 viruses with which it works. well, somehow it's all treated. if anything, if there is some kind of bad story going on somewhere in agriculture. as a rule, we develop complex preparations. we have a separate line. this is an environmentally friendly development safe drugs as alternatives to antibiotics development of antiviral drugs. these are based drugs. immunoglobulins, uh, nanoparticles of lactoferin bioelements, that is, i can’t help asking such a drug plan, but recently. eh, we often hear that on one farm it is on another. uh, you go cattle the reason may be different. but you can turn to you and somehow

you can, well, at least stop this process, one of the services provided by the institute for livestock farms. this scientific and practical assistance. livestock farms often turn to us on various problematic issues. in response to these appeals, the institute sends specialists who oversee various areas. this is an infectious profile or parasitic disease. obstetric gynecological diseases metabolic disorders of the limb disease and e, to establish the causes of non -productive being. our specialists evaluate the episodic situation, look at the state of feeding of animals on the farm and in accordance with this, a selection of biological material is carried out, a laboratory study is carried out to confirm the diagnosis and has already received. uh, some results next. we give appropriate recommendations, rectify vaccination schemes for therapeutic and prophylactic treatments. that is, act here in this problem according to the situation

, i understand correctly, of course, each time the situation is different, so a creative approach is needed here. and there are some. well, let's say the questions that are relevant constantly have them. well , there are such permanent issues to solve annually, and you know in general a viral infection. she, let's say , strives to constantly circulate in the animal population, and for this, nature has developed various mechanisms. that is, the virus can become chronic and then become more active periodically outbreaks of the disease. that's the way. we must adapt to these mechanisms, study them and develop, respectively, preventive and treatment measures that will allow us to provide. better countries, but animals have some kind of seasonal history, here people there in the fall are standard sars influenza. yes , covid. e now, but in the spring the same story, but with animals it's the same. yes? of course, all diseases are seasonal. it is usually

rather the beginning of spring. uh, the end of autumn , various viral infections are also activated, bacterial infections are layered, and animals, naturally, get sick and already appear, uh, clinical symptoms, on which we must already respond. thank you very much. very interesting. we don't say goodbye, i remind you look at lukomania today, study the methods of work of modern aybolites and look further. in a good mood and with a smile on his face. the main rule for creating cells. and viruses sounds and animals. why scientists are increasingly using cellular materials and new research in the field of veterinary medicine for experiments, we will talk about them very soon. the study of animal viruses is now almost impossible without the creation of cells, such a method does not violate ethical norms and gives accurate

results i was intrigued, then i won’t do it anymore torment me in the studio, head of the department of cultures, cells and nutrients of the institute of experimental veterinary medicine natalya ivanovna good afternoon. good afternoon natalya ivanovna kostyuk head of the department of cell cultures and nutrients of the institute of experimental veterinary medicine of the national academy of sciences of belarus candidate of veterinary sciences associate professor natalya ivanovna well, for starters. tell us how you grow cells in general, what biomaterial you use, and how it all looks like. so this is a lab. test tubes. as a cell culture, these are cells of multicellular organisms that live and multiply in artificial conditions, a cell culture can be obtained from almost any organ or tissue, or grown separately in a test tube mattress. as the principle itself occurs, most often it happens in the conditions of the meat processing plant, we get the organ that we need. there's a piece of liver skin and so on. well, for this there is no

need to kill animals. this is all done under very asymptomatic sterile conditions. in the laboratory, tissue is crushed and there is a special solution, under the action of which the cells leave the organs and pass into the solution. this is a very long monotonous process, very complex, but , in principle, master it. any person can. if you like it, such work is further for these cells. it is necessary to create conditions. the main thing is the substrate to which the cells must attach, where they will grow and multiply, a nutrient medium and warmth are required in order to grow cell cultures, i am special environment, let's say environment 199. it contains more than 60 components. there are all vitamins, 17 amino acids, proteins, minerals. this is a rather very complex medium in its composition, which in this nutrient medium and in warmth always needs a temperature of 37-37 °. well, the same as in

animals and in our country. we work at the institute. mostly with transfused cell lines. they are also called immortals, that is, these cells were once received from organs long ago. o created special storage conditions for them when they retrieve from these storage conditions. they feel great and move on. we have in the park cells received in '78. eighties ninety- six and we are successfully extracting them from cryostorage and working with these cells. you said that cryostorage is one of the conditions - low temperature. i'm right, like this, this is a bank in general e cells, what is it and what are the conditions? here, besides the low temperature, the banks have quite a lot of our vessel goes jura, in which liquid nitrogen is poured, the temperature of which is -196 ° and there cells are stored in such a frozen state, and

they are stored for a very long time, they are also called immortal, that is, any moment you take them out of the bank, you create special conditions, and they feel great and come to life. yes , we recently raised the line of 1986, and it still feels great with us and is growing in the present moment. you said to grow a cell. uh, maybe everyone. the main thing is to like working, but i know that there must be a good mood. or is it a myth, really, she feels. we, when we go to work with cells all sorrows, we leave behind the doors of our boxes. and when you sit down your work from laminara, you really forget about a bad mood, well-being, and so on, in general, this is not very, of course, such, as if it were a myth, there were cases when we have, well, a lot of young mothers, that's when de - the cell fell ill and ceased to produce a large crop. well, it has been for some period of time we have noticed it. and now we have

each employee has his own favorite cell for one reason or another, someone likes it, outwardly someone that it gives a big harvest for virology. now let's move on to the results of your labors. yes, use now, uh, the issue of bioethics of animals is becoming more and more relevant. that is why you work closely, there are virologists. and now the whole thing is also happening at the cellular level, not only. here, of course, has contributed. back in the fifties , when she began to develop in virology. at the same time, cell culture began to develop, and when the russian scientist realized that it was possible to work on a cell, to see how the virus interacted with the cell cell culture began to develop and the first vaccine against polio was obtained in the fifty-fourth year, thanks to a virologist , and this vaccine was obtained on cells and even the nobel prize was received, for this vaccine, of course, bioethics contributes. let

's say that in order to diagnose a virus from one or another , a lot of laboratory animals are needed, for example, to make a diagnosis. well, about 25-30. there mice rabbits. there are viruses that do not reproduce in laboratory animals. them. you need yours. that is, as here is the owner, there is a piglet and so on, so here is a very good cell replacement instead of animals in the process of observing how the virus works? we already directly see the cell not on the body, how it happens, but precisely in the cells, of course, at the cellular level, and another plus in the cells is that they are not able to fight the virus, they do not, they do not produce antibodies, and therefore the result is much more effective . what projects, maybe, uh, what are you working on right now, maybe something in priority. yes, we are currently preparing the invitro platform for non-clinical drug research. we are currently selecting.