the thalamus, the hypothalamus and the basoganglia were all destroyed in that first shot.she was incapable of squeezing the trigger a second time. >> the fact that she had two contact gunshot wounds to the head, each of which would be instantly incapacitating and nearly instantly fatal, were sufficient for me to conclude that this was a homicide and not the suicide, as it appeared to be. >> but if david duyst shot his wife two times at close range, why was there no blood spatter on his shirt? >> when the clothing was sent over, it was labeled as a suicide, not a homicide investigation. they just basically held it up and looked at it with a naked eye. >> the clothing was examined again. this time, criminalists used a high-powered microscope connected to a video camera. under high magnification, there were stains invisible to the naked eye, tiny, microscopic bloodstains. >> these stains were referred to as back spatter. they're coming from the entry wound, and they're actually ejected back towards the shooter. >> the blood penetrated into the fabric weave, the signature sign

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